Moccasin Gap is a gap in Clinch Mountain near Gate City, Virginia, United States, the county seat of Scott County. Daniel Boone's Wilderness Road once traveled through the gap, on its way north from the Long Island of the Holston River, located to the south in Tennessee, towards the well-known Cumberland Gap at the boundary of Kentucky, Tennessee, and Virginia.
Moccasin Gap is the more dramatic of only two true, natural gaps in Clinch Mountain, but it is planned to undergo major mountain removal to accommodate a planned highway. The natural appearance of the gap will be altered significantly.